/Length 1063 >> The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". endstream 3 0 obj The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. This is a rapid and reversible staining used for locating protein bands on Western blots and comparing protein band intensity. Kits for reversible protein visualization and quantitation on protein gels and western blot membranes by labeling with near-infrared CF dyes. endobj x3R235W(*T0PR0T(Y@@QC= P AJ 1WpW We also use third-party cookies that help us analyze and understand how you use this website. 35 0 obj All payment in US dollars must be payable on a US bank. This does not affect product stability or performance. <> Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes <> Should I request a replacement? Maleimides, MTS and thiosulfate dyes <> <> Buffer system optimized for fluorescence-based western provides superior specificity, sensitivity, and background suppression. This cookie is set by GDPR Cookie Consent plugin. Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. 5 0 obj <> A solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining. xVmo6__y|'MbpUqg["d}=2\RV2.(84eT{:+.m\"tKd*4P!?/k\a@R'#*`p&LqOpFU+)p.FQ"(${(L(j/-3 endstream Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. <> A note on CF dye succinimidyl ester stability ; fI+j2]Q*oNAVi#F*kNx?[W"Win]tcPqQ2/bINwm"b"]\?X[t}azsAYL@X"6*4(*R/ \HQ0 gu4PzwtQbCn+Z}hA|U0N:nLjuUWwl 7oe}DD:3fU- ^d~y/DtgAo|V1a8`2#(Nr5z Analytical Chemistry (2016), doi:10.1021/acs.analchem.6b00699, Mix-n-Stain CF Dye Antibody Labeling Kits, TrueBlack Lipofuscin Autofluorescence Quencher, Scaling Down to Go Big: An Overview of the Lab-on-a-Chip, Optical Imaging in Tissue with Near-Infrared Dyes, Bring Out Your Dead: v-PCR for Microbial Population and Diversity Studies, Receive 20% Off Your Next Order by Subscribing to Our E-Newsletter, Biotium implements a Quality System, certified by QAS according to Standard QAS ISO 9001:2015. Heavily sulfonated dyes, such as the Alexa Fluor dyes, DyLight dyes and IRDyes are particularly hygroscopic, worsening the hydrolysis problem. endobj The cookie is used to store the user consent for the cookies in the category "Analytics". The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. 16 0 obj Certificate No. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced. This website uses cookies to improve your experience while you navigate through the website. You also have the option to opt-out of these cookies. Some products are shipped with blue ice packs as an extra precaution against high temperatures. endobj The blue ice packs may be thawed upon arrival without affecting product performance. Esters are not stable in aqueous solution. endobj x3R235W(*T0PR0T(Y@@QC= P AJ PpW stream <>/Font <>/ProcSet [/PDF /Text ] Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining. 6 0 obj However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. endobj Coelenterazines are stable for up to five hours in aqueous solution. x3R235W(*T0PR0T(Y@@QC= P AJ 1UpW endstream Ponceau S staining is fast, easy and does not interfere with downstream western blotting. x3R235W(*T0PR0T(Y@@QC= P AJ 1RpW The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes. endstream For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). x3R235W(*T0PR0T(Y@@QC= P AJ 1VpW This cookie is set by GDPR Cookie Consent plugin. A red visible dye that rapidly stains proteins on western blotting membranes for easy visual inspection of protein transfer. Chemicals, dyes, and gel stains Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. Expiration date based on date of manufacture (DOM) Products with recommended storage at ultra low temperature (-70C) that also ship on dry ice should arrive frozen. Azure Ponceau is a reversible stain that detects total protein on both nitrocellulose and PVDF membranes. D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. stream endstream stream Bioscience kits <> Most of our products are stable at room temperature for many days, but we recommend storage at 4C or -20C to prolong shelf life. Diacetate-modified dyes, like ViaFluor 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes. Blog: Your Go-To Guide for Protein Stains, Expiration date based on date of manufacture (DOM), Products with recommended storage at ultra low temperature (-70C) that also, ship on dry ice should arrive frozen. To measure transferred protein quantitatively in fluorescent western blotting, we recommend the VersaBlot Total Protein Normalization Kits. <> Ester dyes are stable in solid form as long as they are protected from light and moisture. endstream x3R235W(*T0PR0T(Y@@QC= P AJ 1QpW x3R235W(*T0PR0T(Y@@QC= P AJ 1SpW endstream Where can I find the expiration date or shelf life of a product? Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit (50 Preps), Minute Detergent-Free Single Nuclei Isolation Kit (20 Preps), Minute Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps), Minute Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps), Minute Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps), Minute Detergent-Free Protein Extraction Kit for Animal Tissues and Cultured Cells(50 Preps), Minute Total Protein Extraction Kit for Plant Tissues (50 Preps), Minute Detergent-Free Plant Protein Extraction Kit (50 Preps), Minute Total Protein Extraction Kit for Mass Spectrometry (50 Preps), Minute Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps, Minute Bacterial Total Protein Extraction Kit (50 Preps), Minute Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps), Minute Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps), Protein Extraction Kit for Hair and Nails (20 Preps), Minute Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps), Minute Total Protein Extraction Kit for Skin Tissue (50 Preps), Minute Total Protein Extraction Kit for Bone Tissue (50 Preps), Minute Total Protein Extraction Kit for Blood Vessels (50 Preps), Minute Total Protein Extraction Kit for Tendons (50 Preps), Minute Total Protein Extraction Kit for Intestines and Mesentery (50 Preps), Minute Total Protein Extraction Kit for Muscles (50 Preps), Minute Total Protein Exatraction Kit for Insects (50 Preps), Minute Histone/DNA Binding Protein Extraction Kit (50 Preps), Minute Protein/Nucleic Acid Extraction Kit from Gels (20 Preps), Minute Adipose Tissue Fractionation Kit (20 Preps), Minute Chloroplast Isolation Kit (50 Preps), Minute Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps), Minute Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps), Minute Cytosolic Proteasome Enrichment Kit (20 preps), Minute Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps, Minute Endosome Isolation and Cell Fractionation Kit (20 Preps), Minute Golgi Apparatus Enrichment Kit (20 Preps), Minute High Fidelity ER Isolation Kit for Liver Tissue (20 Preps), Minute High-Efficiency Exosome Precipitation Reagent (20 ml), Minute High-Efficiency Saliva Exosome Isolation Kit (50 Preps), Minute Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells, Minute Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps), Minute Nuclear Proteasome Enrichment Kit (20 preps), Minute Nuclear Envelope Protein Extraction Kit (50 Preps), Minute Plant Cytosolic and Nuclear Protein Isolation Kit (Need 150 mg Raw Material, 20 preps), Minute Plant Endosome Enrichment Kit (20 preps), Minute Plant ER Enrichment Kit (20 preps), Minute Plant Golgi Apparatus Enrichment Kit (20 preps), Minute Plant Lipid Raft Isolation Kit (20 preps), Minute Plant Microsomal Membrane Extraction Kit (50 Preps), Minute Plant Plasma Membrane Protein Isolation Kit (50 Preps), Minute Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps), Minute Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues, Minute Yeast Mitochondria Enrichment Kit (50 Preps), Minute Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps), Minute Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps), Minute Detergent-Free Nuclei Isolation Kit (20 Preps), Minute Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps), Minute Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps), Minute Single Nucleus Isolation Kit for Tissues/Cells, Minute Native Protein Precipitation Kit from Saliva, Minute Organic Solvent-Free Milk Lipid Depletion Kit, Minute Albumin Depletion Reagent for Plasma and Serum (20 ml), Minute Denaturing Protein Solubilization Reagent (10 ml), Minute High-Efficiency Protein Precipitation Kit (30 ml), Minute Non-Denatured Protein Solubilization Reagent (10 ml), Minute Protein Solubilization Reagent for MS (2 ml), Minute Western Blot Stripping Solution (250 ml). Kits often are functional for significantly longer than the guaranteed shelf life. Find, Sign up for exclusive offers and be the first to know about upcoming products. The staining is reversible and membranes can be used immediately for Western blotting after de-staining. We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits.

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