Be the first to To download a Certificate of Analysis, please select a batch below. Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. (1986). Your browser does not have JavaScript enabled and some parts of this website will not work without it. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). 30 ug of protein was loaded per well and image was taken using a LiCor Odyssey imager The staining worked but the signal was not as strong as other Ponceau S stains I have used before. representative of CST, are rejected and are of no force or effect. There are no specific FAQs related to this product. If desired, mark proteins with waterproof ink or a pencil. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Buffers, Chelators and Reagents Staining Kits and Reagents, View Full Technical Details for Ponceau S Staining Solution. All Rights Reserved. Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. This product is for research use only and is not approved for use in humans or in clinical diagnosis. The reviewer received a reward from Abcams Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions. Customer shall not use any Product for any diagnostic Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Wash membrane in ddH2O until distinct reddish-pink protein bands are visible (1-5 min). Take a look at our BETA site and see what weve done so far. Ponceau red stain is not stable and will disappear if you destain longer.3. Agonists, activators, antagonists and inhibitors, Anti-CYP2C9 antibody [EPR7340] (ab150364). Would you like to visit your country specific website? Tocris products are intended for laboratory research use only, unless stated otherwise. Tocris Bioscience is the leading supplier of novel and exclusive structure or technology of the Products, or use the Products for the purpose of developing any products or services that would *When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). 1. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. TBS/BSA). Get resources and offers direct to your inbox. References are publications that support the biological activity of the product. For batch specific data refer to the Certificate of Analysis. The technical data provided above is for guidance only. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed Tocris offers the following scientific literature in this area to showcase our products. ACD branded products will be available on biotechne.com in the near future. This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms requires a separate license from CST. FAX 612 656 4400. apply to Products provided by CST, its affiliates or its distributors. vial. Following protein electrotransfer, rinse membrane briefly in ddH2O to remove any detergent that may inhibit staining. We have publications tested in 1 application: WB. 1. To get a clear background, wash the membrane quickly in PBS after staining2. CST Product Terms of Sale and any applicable & Earn an Amazon Gift Card, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution), TSA Reagents for Enhancing IHC, ICC & FISH Signals. It was previously called Ponceau S Solution - 1L. compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections. *Please note that Tocris will only send literature to established scientific business / institute addresses. View all citations for the Staining Kits and Reagents platform. USA, TEL 612 379 2956 We offer a 100% guarantee on all our products. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Tocris is a Bio-Techne brand. Store at Room Temperature. 6. To get a clear background, wash the membrane quickly in PBS after staining2. tools for Visit our, Salinovich, O. and Montelaro, R.C. Calculate the dilution required to prepare a stock solution. Azide-containing monosaccharide building block; used as glycoprotein, glycolipid and glycoRNA probe, Amine reactive biotinylation reagent, cell permeable. We invite you to request* your copy today! If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Copyright 2022 R&D Systems, Inc. All Rights Reserved. Bacterial peptidoglycan derivative; suitable for 'click'-conjugation to fluorescent dyes. This product is manufactured by Expedeon, an Abcam company. Expedeon product code 5999-1L is the same as the1000 mL size of this product. Minneapolis, MN 55413 The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. Please note: All products are "FOR RESEARCH USE ONLY. Note: Mouseover a species abbreviation on the product page to display the fullname. Store at room temperature. Purchase these through your usual distributor. the vial label and CoA (available online). Read our general, There are no specific blogs for Ponceau S Staining Solution, but you can. If you know of a relevant reference for Ponceau S Staining Solution, please let us know. No. copyright notices or markings, (d) use the Products solely in accordance with review Ponceau S Staining Solution and earn rewards! Mar 23 2019 [PMID: 30914243] (WB), Review with no image -- $10/7/6/$10 CAD/70 Yuan/1110 Yen, Review with an image -- $25/18/15/$25 CAD/150 Yuan/2500 Yen. Please contact us to place your order, or try again later. 3. reviews that match your criteria. Incubate membrane in Ponceau S Staining Solution for 5-10 minutes at room temperature. We use Ponceau S (Cat. Calculate the mass, volume, or concentration required for a solution. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying The intensity of staining corresponds to the amount of protein transferred. Any Customer's terms and conditions that are in All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce background staining on the blotting membrane. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Stain used to detect protein bands after Western blotting, 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Support products are. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, Explore the power of knock-out cell lines for your research. The technical data provided above is for guidance only. by the FDA or other regulatory foreign or domestic entity, for any purpose. There are no reviews for Ponceau S Staining Solution (NB5225). Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Get the latest news, product updates, and promotions: Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.. Best staining for tracking protein transfer in western blot. decide on the best products for their research. For batch specific data refer to the Certificate of Analysis. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any As little as 250 ng protein can be rapidly visualized with Ponceau S stain. Shipped at Room Temperature. Copyright 2022 Bio-Techne. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Get the latest news, product updates, and promotions: Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Do not dilute or aliquot. Products sold or licensed by CST review today?! 4. Please let us know so that we can cite the reference in this datasheet. 2022 Novus Biologicals, All Rights Reserved. Ponceau red stain is not stable and will disappear if you destain longer.3. Fluorescent Probes and Dyes Product Guide, Citations for Ponceau S Staining Solution, GMP & Ancillary Material Grade Small Molecules, Certificate of Analysis / Product Datasheet, Submit a Review By continuing to use this website you acknowledge this and agree to our cookie policy. Toll-free 800 343 7475 Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. Ponceau S waste: Ponceau S staining for total protein normalization Anal. Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 g BSA, lane 5 1 g BSA, lane 6 500 ng BSA, lane 7 250 ng BSA. review will help other researchers Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience. Tocris products are intended for laboratory research use only, unless stated otherwise. ), $50/35/30/$50CAN/300 Yuan/5000 Yen for first to review with an image, $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. This ponceau S solution was used to stain total protein that had been applied to PVDF membrane using during a slot blot experiment. Average Rating: 4.3 (Based on 3 Reviews. Alternatively, a photograph of the stained membrane can also be taken. ProteinSimple branded instruments are available to quote. Citations are publications that use Tocris products. There are currently no reviews for this product. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. View all citations for the Staining Kits and Reagents platform. Bio-Techne uses cookies to provide you with a great website experience. There are no Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE For batch specific data refer to the Certificate of Analysis. We use Ponceau S (Cat. Currently there are no citations for Ponceau S Staining Solution. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or Guaranteed product quality, expert customer support. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. You can now submit reviews for your favorite Tocris products. industry. prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, No. 5. Following staining, membranes can be de-stained with any buffer of pH >7 or by transfer of the membrane into blocking buffer (e.g. Changing to another country might result in loss of shopping cart. or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Our guide highlights the use of Fluorescent Probes and Dyes in research and provides background information on various imaging techniques. Be the first to review our Ponceau S Staining Solution and receive a gift card or discount. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This product is stable for 12 months. Ponceau S Staining Solution Support Products, View other related Ponceau S Staining Solution Support Products, Sander H, Wallace S, Plouse R et al. NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). 1. Your Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. This enables one to incubate the different membrane sections with various primary antibodies. Tocris products are intended for laboratory research use only, unless stated otherwise. rest. life science research with over 30 years' experience in the Biochem. No product specific FAQs exist for this product, however you may. Which Brands are Currently Available on biotechne.com? Wash membrane in 1X TBS-T several times for 5 min at room temperature until protein bands are no longer visible. Any use of Product for diagnostic, $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. Proceed with blocking step of Western blot procedure. ab270042 has not yet been referenced specifically in any publications. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. Simply enter the mass of reagent and the target concentration and the calculator will determine the 2. Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. *When preparing stock solutions always use the batch-specific molecular weight of the product found on Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. This enables one to incubate the different membrane sections with various primary antibodies. Why not submit a All rights reserved. Products in stock will be shipped in 1-3 business days unless specified. are provided for Customer as the end-user and solely for research and development uses. Please let us know. Constituents: 0.1% Ponceau S, 0.96% Acetic acid. Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). Publishing research using ab270042? The technical data provided above is for guidance only. 1998-2022 Abcam plc. services used by Customer in connection with the Products.

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